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Epicypher新品 | 核酸提取缓冲液

2023-11-22
浏览次数: 62


Epicypher新品 | 核酸提取缓冲液


CUTANA™ 细胞核提取缓冲液是CUT&RUN和CUT&Tag实验中,用于从培养的细胞和组织中提取细胞核的必需试剂。这种缓冲液可与多种真核细胞和组织兼容。以下是使用该缓冲液成功进行细胞核分离的细胞和组织类型:

mouse NIH3T3 fibroblast cells

human A549 non-small cell lung cancer (NSCLC) cells

human K562 leukemia cells

human NCI-H1299 non-small cell lung cancer (NSCLS cells)

human bone marrow derived macrophages

human TIG-1 fetal lung cells

human monocyte derived macrophages

human LoVo colorectal cancer cells

human MV-4-11 macrophage cells

human LNCaP prostate carcinoma cells

human SUM149 triple negative breast cancer (TNBC) cells

human renal primary cells

human GM24385 B-lymphocyte (aka HG002) cells

human peripheral blood mononuclear cells (PBMCs)

human MCF7 breast cancer cells

human intestinal tissue

human MDA-MB-231 breast cancer cells

human HEPM embryonic cells

human SK-MEL-2 melanoma cells


 

只需将试剂盒中的Pre-Nuclei Extraction Buffer和亚精胺与蛋白酶抑制剂混合即可完成该试剂的制备。CUTANA™ 细胞核提取缓冲液针对CUT&RUN和CUT&Tag实验流程进行了优化,从而获得高质量的细胞核,助力您的基因组图谱分析。

 

试剂盒组成

组分

货号

CUTANA™ Pre-Nuclei Extraction Buffer      

21-1026a     

1 M Spermidine

21-1026b


保存条件

将Pre-Nuclei Extraction Buffer 4ºC储存, 1 M亚精胺-20ºC储存。

自收到之日起可稳定储存6个月。


验证数据

Epicypher新品 | 核酸提取缓冲液

Figure 1: Nuclei Extraction

Nuclei were extracted from 3 different cell types: K562, NIH3T3, and LNCaP using the CUTANA™ Nuclei Extraction Protocol for CUT&RUN and CUT&Tag. Top 3 panels show cells before extraction. Starting cells are viable (bright white and round). Bottom 3 panels show nuclei extracted using the CUTANA™ Nuclei Extraction Buffer as indicated by positive Trypan Blue staining.


订购详情

货号

产品名称

规格

21-1026

CUTANA™ Nuclei Extraction Buffer

100 Reactions

 



Epicypher热销产品——CUTANA™ pAG-Tn5 for CUT&Tag

如需了解更多详细信息或相关产品,请联系EpiCypher中国授权代理商-欣博盛生物 

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