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ROS-ID®活性氧/活性氮检测试剂盒——ENZO热销产品

2023-10-23
浏览次数: 61

自由基和其他活性种在许多心理和病理生理过程中发挥着重要作用。自由基一旦在细胞内产生,就会破坏多种细胞成分,包括蛋白质、脂质和DNA。然而,在较低浓度下,自由基可以作为细胞信号传导中的第二信使。

Enzo Life Sciences的ROS-ID® ROS/RNS detection kit可用荧光显微镜直接实时监测活细胞中活性氧与活性氮(ROS/RNS)的产生。该试剂盒中包含三种检测探针:NO检测试剂(红色,Ex/Em 650/670 nm),用于总ROS检测的氧化应激检测试剂(绿色,Ex/Em 490/525 nm)和超氧化物检测试剂(橙色,Ex/Em 550/620 nm)。试剂盒内还包括阳性对照,绿脓菌素和L-精氨酸,它们分别是ROS和NO产生的常见诱导剂;阴性对照N-乙酰-L-半胱氨酸和c-PTIO则分别是ROS和NO的常见清除剂。通过将三种检测探针与一组特异性抑制剂、激活剂相结合,能够区分超氧化物、一氧化氮和过氧亚硝酸盐。

 

产品特点

● 直接监测活细胞中的活性氧和氮化物(ROS/RNS)

● 可区分超氧化物、一氧化氮和过氧亚硝酸盐

● 高灵敏度、特异性和准确性,可用于活细胞研究

● 与组织培养基的主要成分(酚红、FBS和BSA)兼容

● 试剂盒内包含整套试剂,包括ROS/RNS诱导剂和清除剂

 

实验示例

ROS-ID®活性氧/活性氮检测试剂盒——ENZO热销产品

图1. 氧化应激检测试剂(绿色)、超氧化物检测试剂(蓝色)和NO检测试剂(红色)的激发和发射光谱。

 

ROS-ID®活性氧/活性氮检测试剂盒——ENZO热销产品

图2. 使用ROS/RNS检测试剂盒演示HeLa细胞中过氧亚硝酸盐和NO生成的代表性实验。分析表明,由于NO与超氧化物的反应,L-精氨酸和绿脓菌素的联合处理产生过氧亚硝酸盐(绿色荧光),但很少产生NO(红色荧光)。

 

ROS-ID®活性氧/活性氮检测试剂盒——ENZO热销产品

  

产品信息

产品货号

ENZ-51001-200

产品名称

ROS-ID® ROS/RNS detection kit

别名

Reactive oxygen species / Reactive nitrogen species

规格

1*1Kit

长期保存

-80°C

试剂盒组分

NO Detection Reagent (Red), 60µl

Oxidative Stress Detection Reagent (Green), 300 nmoles

Superoxide Detection Reagent (Orange), 300 nmoles

NO Inducer (L-Arginine), 100 µl

ROS Inducer (Pyocyanin), 1 µmole

NO Scavenger (c-PTIO), 400 nmoles

ROS Inhibitor (N-acetyl-L-cysteine), 2x10 mg

10X Wash Buffer, 15 ml

应用

Fluorescence microscopy

 

部分产品引用文献

1. Hypoxia-induced oxidative stress promotes therapy resistance via upregulation of heme oxygenase-1 in multiple myeloma: K. Abe, et al.; Cancer Med. (2023)

2. Dihydroartemisinin-transferrin adducts enhance TRAIL-induced apoptosis in triple-negative breast cancer in a P53-independent and ROS-dependent manner: X. Zhou, et al.; Front. Oncol. 11, 789336 (2022)

3. Intermittent exposure of cultured endothelial cells to physiologically relevant fructose concentrations has a profound impact on nitric oxide production and bioenergetics: M.L. Fiorello, et al.; PLoS One 17, e0267675 (2022)

4. S1P (Sphingosine-1-Phosphate)-Induced Vasodilation in Human Resistance Arterioles During Health and Disease: B. Katunaric, et al.; Hypertension 79, 2250 (2022)

5. Extra-mitochondrial citrate synthase initiates calcium oscillation and suppresses age-dependent sperm dysfunction: W. Kang, et al.; Lab. Invest. 100, 583 (2020)

6. Liposome-encapsulated bacillus calmette-guérin cell wall skeleton enhances antitumor efficiency for bladder cancer in vitro and in vivo via induction of amp-activated protein kinase: Y.M. Whang, et al.; Cancers (Basel) 12, 3679 (2020)

7. Mesenchymal stem cell conditioned medium ameliorates diabetic serum-induced insulin resistance in 3T3-L1 cells: A. Sanap, et al.; Chronic Dis. Transl. Med. 7, 47 (2020)

8. Isoorientin derived from Gentiana veitchiorum Hemsl. flowers inhibits melanogenesis by down-regulating MITF-induced tyrosinase expression: Q. Wu, et al.; Phytomedicine 57, 129 (2019)

9. Low intensity ultrasound reduces high glucose reduced nitric oxide generation in retinal pigment epithelial cells: M.B. Karmacharya, et al.; Ultrasound Med. Biol. 44, 647 (2018)

10. HEK-293 secretome attenuates kainic acid neurotoxicity through insulin like growth factor-phosphatidylinositol-3-kinases pathway and by temporal regulation of antioxidant defense machineries: C. Venugopal, et al.; Neurotoxicology 69, 189 (2017)

11. Selective enhancing effect of metal ions on mutagenicity: N. Fujii, et al.; Genes Environ. 38, 21 (2016)

12. Cell-to-cell diffusion of glucose in the mammalian heart is disrupted by high glucose. Implications for the diabetic heart: W.C. De Mello ; Exp. Cell Res. 334, 239 (2015)

13. Increased Proinflammatory Cytokine Production and Decreased Cholesterol Efflux Due to Downregulation of ABCG1 in Macrophages Exposed to Indoxyl Sulfate: K. Matsuo, et al. ; Toxins (Basel) 7, 3155 (2015)

14. Regulation of gene expression by tobacco product preparations in cultured human dermal fibroblasts: G.E. Malpass, et al.; Toxicol. Appl. Pharmacol. 279, 211 (2014)

15. Enterococcus faecalis infection causes inflammation, intracellular oxphos-independent ROS production, and DNA damage in human gastric cancer cells: J.A. Strickertsson, et al.; PLoS One 8, e63147 (2013)

 

 

 

Epicypher热销产品——CUTANA™ pAG-Tn5 for CUT&Tag

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