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GFP-CERTIFIED®FLUOFORTE®钙离子检测试剂盒——ENZO热销产品

2023-10-23
浏览次数: 17

Enzo Life SciencesGFP-CERTIFIED® FLUOFORTE® Calcium assay kit提供了一种荧光分析方法,用于检测广泛生物靶标的细胞内钙动员情况。相对于其他商业化的染料,GFP-CERTIFIED® FLUOFORTE®染料是最亮和最灵敏的荧光钙指示剂该试剂盒为贴壁和非贴壁细胞提供了更少步骤,更低变异性,更简易钙检测。此外,它既不需要洗涤步骤,也不需要添加外源性淬灭染料,避免受体-配体相互作用动力学的干扰。

Enzo Life Sciences的GFP-CERTIFIED® FLUOFORTE® Calcium assay kit可用于细胞内钙离子的高通量检测,钙离子是重要的第二信使,常用于初步筛选中监测G蛋白偶联受体激活。

 

产品特色

● 可监测表达GFP细胞中的钙离子动员情况

● 最明亮、最灵敏的荧光Ca2+指示剂

● 获得的 EC50和IC50值与使用钙离子荧光探针 Rhod-4 AM和Fluo-4 AM 相当

● 更大范围的检测窗口

● 使用方便,无需洗涤,无需淬灭染料

 

实验示例

GFP-CERTIFIED®FLUOFORTE®钙离子检测试剂盒——ENZO热销产品

 

GFP-CERTIFIED®FLUOFORTE®钙离子检测试剂盒——ENZO热销产品 

2. U-2 OS细胞分别与(AGFP-CERTIFIED® FLUOFORTE®AM染料BRhod-2 AM染料孵育。洗涤细胞并用终浓度为100nMATP处理,并立即成像。GFP-CERTIFIED® FLUOFORTE® AM染料比Rhod-2 AM表现出更亮的荧光强度。

 

产品信息

产品货号

ENZ-51020-KP010/ ENZ-51020-KP100

产品名称

GFP-CERTIFIED® FLUOFORTE® Calcium assay kit

规       格

10*96wells/100*96wells

短期保存

-20°C

长期保存

-80°C

试剂盒组分

Starter pack (Prod. No. ENZ-51020-KP010):

Reagent A (lyophilized GFP-CERTIFIED® FLUOFORTE® dye), 1 vial

Reagent B (dye efflux inhibitor), 10ml

Reagent C (Hanks’ buffer with 20mM HEPES, 100ml)

Sufficient for 10 x 96-well plates


High-throughput (Prod. No. ENZ-51020-KP100):

Reagent A (lyophilized GFP-CERTIFIED® FLUOFORTE® dye), 10 vials

Reagent B (dye efflux inhibitor), 10 x 10ml

Sufficient for 100 x 96-well plates

 

部分产品引用文献

1. Perfluorooctane sulfonate and bisphenol A induce a similar level of mast cell activation via a common signaling pathway, Fyn-Lyn-Syk activation: S.J. Park, et al.; Food Chem. Toxicol. 156, 112478 (2021)

2. horbol myristate acetate induces differentiation of THP-1 cells in a nitric oxide-dependent manner: Y.Y. Chang, et al.; Nitric Oxide 109, 33 (2021)

3. Absolute proteomic quantification reveals design principles of sperm flagellar chemosensation: C. Trötschel, et al.; EMBO J. 39, e102723 (2020)

4. Nanobody-directed targeting of optogenetic tools to study signaling in the primary cilium: J.N. Hansen, et al.; Elife 9, e57907 (2020)

5. Interplay between sub-cellular alterations of calcium release and T-tubular defects in cardiac diseases: M. Scardigli, et al.; Front. Physiol. 9, 1474 (2018)

6. Characterization of cholesterol homeostasis in sphingosine-1-phosphate lyase-deficient fibroblasts reveals a Niemann-Pick disease type C-like phenotype: H. Vienken, et al.; Sci. Rep. 7, 43575 (2017)

7. Novel insights on the relationship between T-tubular defects and contractile dysfunction in a mouse model of hypertrophic cardiomyopathy: C. Crocini, et al.; J. Mol. Cell. Cardiol. 91, 42 (2016)

8. Sulfur dioxide protects against collagen accumulation in pulmonary artery in association with downregulation of the transforming growth factor β1/smad pathway in pulmonary hypertensive rats: W. Yu, et al.; J. Am. Heart Assoc. 5, e003910 (2016)

9. T-tubular electrical defects contribute to blunted β-adrenergic response in heart failure: C. Crocini, et al.; Int. J. Mol. Sci. 17, 1471 (2016)

10. Defects in T-tubular electrical activity underlie local alterations of calcium release in heart failure: C. Crocini, et al.; PNAS 111, 15196 (2014)

 

 


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