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Epicypher热销产品——CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows

2023-09-17
浏览次数: 28

CUTANATM pAG-MNase是进行染色质免疫切割(ChIC)和核酸酶靶向切割和释放(CUT&RUN)的关键试剂。作为蛋白A/G与微球菌核酸酶的融合表达产物,CUTANA pAGMNase与来自各种物种宿主的靶抗体兼容,并且经过高度纯化以去除污染的E. coli DNA,这可能会使细胞数量少的样本分析复杂化。与ChIP-seq相比,pAG-MNase可以在ChIC/CUT&RUN中有效地绘制染色质特征,可以显著改善信噪比和测序深度。


保存条件

Stable for one year at -20°C from date of receipt.


数据示例

Epicypher热销产品——CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows

FIGURE 1:

CUT&RUN gene browser tracks. CUT&RUN was performed as described above. Data verifies low non-specific MNase digestion with the absence of peaks in the IgG track, an expected H3K4me3 profile with sharp promoter peaks, and broad peaks in heterochromatin regions consistent with H3K27me3. Image was generated using the Integrative Genomics Viewer (IGV, Broad Institute).

Epicypher热销产品——CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows 

FIGURE 2:

Size distribution of released chromatin. CUT&RUN was performed as described above. Excised DNA is highly enriched for mononucleosomes (peaks at ~300 bp represent 150 bp nucleosomes + sequencing adapters).

Epicypher热销产品——CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows 

FIGURE 3:

CUT&RUN genome-wide heatmaps. CUT&RUN was performed as described above. Heatmaps show CUT&RUN signal aligned to annotated transcription start sites (TSS, +/- 2kb). High and low signal are ranked by intensity (top to bottom) and colored such that red indicates high localized enrichment and blue denotes background signal. Gene rows in each heatmap are aligned and sorted from high to low signal relative to H3K4me3 (middle).

Epicypher热销产品——CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows

FIGURE 4:

Protein gel data. CUTANATM pAG-MNase (1 µg) was resolved via SDS-PAGE and stained with Coomassie blue. The migration and molecular weight of the protein standards are indicated.


订购详情

货号

产品名称

规格

15-1016

CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows

50 Reactions

15-1116

CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows

250 Reactions

 


Epicypher热销产品——CUTANA™ pAG-Tn5 for CUT&Tag

如需了解更多详细信息或相关产品,请联系EpiCypher中国授权代理商-欣博盛生物 

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