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BioXcell热销产品--BE0273 InVivoMab anti-mouse PD-1 (CD279)

2023-04-27
浏览次数: 61

BioXcell热销产品--BE0273 InVivoMab anti-mouse PD-1 (CD279)

 

产品描述:

BioXcell InVivoMab anti-mouse PD-1 29F.1A12单克隆抗体与小鼠PD-1(也称为CD279)反应。PD-1是一种50-55kDa的细胞表面受体,由Pdcd1基因编码,属于Ig超家族的CD28家族。PD-1在CD4和CD8胸腺细胞以及活化的T和B淋巴细胞和髓细胞上瞬时表达,在成功消除抗原后PD-1的表达下降。此外,在B细胞前阶段,Pdcd1 mRNA在发育中的B淋巴细胞中表达。PD-1的结构包括ITIM(基于免疫受体酪氨酸的抑制基序),这表明PD-1负调控TCR信号。PD-1通过结合B7家族的成员PD-L1和PD-L2发出信号。在配体结合后,PD-1信号传导抑制T细胞活化,导致增殖减少,细胞因子产生和T细胞死亡。此外,PD-1敲除动物表现出扩张型心肌病、脾肿大和外周耐受丧失,在小鼠的外周耐受性和预防自身免疫性疾病中发挥关键作用。诱导的PD-L1表达常见于许多肿瘤,包括鳞状细胞癌、结肠癌和乳腺癌。PD-L1过度表达会导致肿瘤细胞对CD8T细胞介导的裂解的抗性增加。在黑素瘤的小鼠模型中,可以通过用阻断PD-L1与其受体PD-1之间相互作用的抗体治疗来暂时抑制肿瘤生长。由于这些原因,目前正在探索抗PD-1介导的免疫疗法作为癌症治疗。与RMP1-14和J43抗体一样,29F.1A12抗体在体内阻断PD-1与其配体的结合。


BioXcell热销产品--BE0273 InVivoMab anti-mouse PD-1 (CD279) 


产品详情:


产品名称

InVivoMAb anti-mouse PD-1 (CD279)

产品货号

BE0273

产品规格

1/5/25/50/100mg

反应种属

Mouse

克隆号

29F.1A12

同种型

Rat IgG2a

免疫原

Recombinant PD-1-Ig fusion protein

实验应用

in vivo blocking of PD-1/PD-L signaling

in vitro PD-1 neutralization

Immunohistochemistry (frozen)

Immunofluorescence

Western blot

Flow cytometry

产品形式

PBS, pH 7.0,Contains no stabilizers or preservatives

纯度

>95%, Determined by SDS-PAGE

无菌处理

0.2 µm filtration

纯化方式

Protein G

RRID

AB_2687796

分子量

150 kDa

保存条件

抗体原液保存在4°C,不能冷冻保存。

推荐同型对照

InVivoMAb rat IgG2a isotype control, anti-trinitrophenol(货号BE0089)

推荐抗体稀释液

InVivoPure pH 7.0 Dilution Buffer(货号IP0070)



该产品自上市已被多篇SCI文献引用,品质有保证,以下是部分已发表的文献引用:


应用

文章

体内PD-1/PD-L信号阻断

(in vivo blocking of PD-1/PD-L signaling)

1. Wang, W., et al. (2018). 'RIP1 Kinase Drives Macrophage-Mediated Adaptive Immune Tolerance in Pancreatic Cancer' Cancer Cell 34(5): 757-774 e757.

2. Gordon, S. R., et al. (2017). 'PD-1 expression by tumour-associated macrophages inhibits phagocytosis and tumour immunity' Nature 545(7655): 495-499.  

3. Koyama, S., et al. (2016). 'STK11/LKB1 Deficiency Promotes Neutrophil Recruitment and Proinflammatory Cytokine Production to Suppress T-cell Activity in the Lung Tumor Microenvironment' Cancer Res 76(5): 999-1008.

体内PD-1/PD-L信号阻断,流式细胞术

(in vivo blocking of PD-1/PD-L signaling, Flow Cytometry)

1.Koyama, S., et al. (2016). 'Adaptive resistance to therapeutic PD-1 blockade is associated with upregulation of alternative immune checkpoints' Nat Commun 7: 10501.

体外PD-1中和

(in vitro PD-1 neutralization)

1.Park, S. J., et al. (2014). 'Negative role of inducible PD-1 on survival of activated dendritic cells' J Leukoc Biol 95(4): 621-629.

体内PD-1/PD-L信号阻断,体外PD-1中和

(in vivo blocking of PD-1/PD-L signaling, in vitro PD-1 neutralization)

1.Duraiswamy, J., et al. (2013). 'Dual blockade of PD-1 and CTLA-4 combined with tumor vaccine effectively restores T-cell rejection function in tumors' Cancer Res 73(12): 3591-3603.

流式细胞术

(Flow Cytometry)

1.Good-Jacobson, K. L., et al. (2012). 'CD80 expression on B cells regulates murine T follicular helper development, germinal center B cell survival, and plasma cell generation' J Immunol 188(9): 4217-4225.

免疫荧光,免疫印迹

(Immunofluorescence, Western Blot)

1.Chen, L., et al. (2009). 'Role of the immune modulator programmed cell death-1 during development and apoptosis of mouse retinal ganglion cells' Invest Ophthalmol Vis Sci 50(10): 4941-4948.

免疫组织化学(冷冻)

(Immunohistochemistry (frozen))

1. Menke, J., et al. (2007). 'Programmed death 1 ligand (PD-L) 1 and PD-L2 limit autoimmune kidney disease: distinct roles' J Immunol 179(11): 7466-7477.

2. Liang, S. C., et al. (2003). 'Regulation of PD-1, PD-L1, and PD-L2 expression during normal and autoimmune responses' Eur J Immunol 33(10): 2706-2716.

 

 

 

 

BioXcell热销产品--BE0273 InVivoMab anti-mouse PD-1 (CD279)

更多产品详情请咨询 BioXcell 中国授权代理——欣博盛生物

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