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Biotin-16-Aminoallyl-2'-dCTP - (N-5002)

2021-11-15
浏览次数: 68

Biotin-16-Aminoallyl-2'-dCTP - (N-5002)又名Biotin-16-dCTP, Biotin-16-AA-dCTP。

Biotin-16-Aminoallyl-2'-dCTP - (N-5002)


产品描述

Biotin-16-Aminoallyl-2'-deoxycytidine-5'-Triphosphate可用于通过PCR、切口平移、使用Klenow(exo-)DNA聚合酶的引物延伸和末端脱氧核苷酸转移酶标记的3'端,将生物素掺入DNA中。可以使用Moloney鼠白血病病毒(M-MLV)逆转录酶与此dNTP制备生物素化的cDNA。


产品信息

货号

N-5002

规格

50 nmole1 µmole

纯度

≥90% by AX-HPLC

保存温度

-20℃或以下

分子式

C32H53N8O17P3S (free acid)

盐离子形式

Li+

消光系数

5,040 Lmol-1cm-1 at 290 nm

溶液

H2O

浓度

5 mM

骨架

5'-三磷酸盐

糖类型

DNA

核苷酸类别

生物素标记

应用

适体、表观遗传学/DNA损伤、体外转录、诱变、光交联研究


文献参考:


Green, Nathaniel M.; Moody, Krishna-Sulayman; Debatis, Michelle; Marshak-Rothstein, Ann . Activation of autoreactive B cells by endogenous TLR7 and TLR3 RNA ligands.


Limbo, Oliver; Moiani, Davide; Kertokalio, Aryandi; Wyman, Claire; Tainer, John A.; Russell, Paul . Mre11 ATLD17/18 mutation retains Tel1/ATM activity but blocks DNA double-strand break repair.


Song, Chun-Xiao; Diao, Jiajie; Brunger, Axel T.; Quake, Stephen R. . Simultaneous single-molecule epigenetic imaging of DNA methylation and hydroxymethylation.


Gangras, Pooja; Dayeh, Daniel M.; Mabin, Justin W.; Nakanishi, Kotaro; Singh, Guramrit . Cloning and Identification of Recombinant Argonaute-Bound Small RNAs Using Next-Generation Sequencing.


Hede, Marianne Smedegaard; Fjelstrup, Søren; Lötsch, Felix; Zoleko, Rella Manego; Klicpera, Anna; Groger, Mirjam; Mischlinger, Johannes; Endame, Lilian; Veletzky, Luzia; Neher, Ronja; Simonsen, Anne Katrine Wrist; Petersen, Eskild; Mombo-Ngoma, Ghyslain; Stougaard, Magnus; Ho, Yi-Ping; Labouriau, Rodrigo; Ramharter, Michael; Knudsen, Birgitta Ruth . Detection of the Malaria causing Plasmodium Parasite in Saliva from Infected Patients using Topoisomerase I Activity as a Biomarker.


Veneziano, Rémi; Shepherd, Tyson R.; Ratanalert, Sakul; Bellou, Leila; Tao, Chaoqun; Bathe, Mark . In vitro synthesis of gene-length single-stranded DNA.


Braun, Joerg E.; Friedman, Larry J.; Gelles, Jeff; Moore, Melissa J. . Synergistic assembly of human pre-spliceosomes across introns and exons.


Ho, Nicholas R. Y.; Lim, Geok Soon; Sundah, Noah R.; Lim, Diana; Loh, Tze Ping; Shao, Huilin . Visual and modular detection of pathogen nucleic acids with enzyme-DNA molecular complexes.


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