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CleanCap® FLuc mRNA - (L-7602)

2021-11-02
浏览次数: 63

产品描述

FLuc mRNA表达了一个最初从萤火虫中提取的荧光素酶蛋白。FLuc在底物荧光素存在下发光,常用于哺乳动物细胞培养用于测检测基因表达和细胞活力。


FLuc mRNA使用了TriLink特有的共转录加帽试剂CleanCap进行加帽,该加帽试剂可产生Cap 1结构,并具有高效的加帽效率。FLuc mRNA是多聚腺苷酸化的,并针对哺乳动物系统进行了优化,模仿完全加工的成熟mRNA。


产品信息:

货号

L-7602

规格

100 µg1 mg5 x 1 mg

纯度

通过琼脂糖凝胶迁移实验

保存温度

-40℃或以下

长度

1929个核苷酸

碱基组成

未修饰的碱基

换算系数

40 µg/OD260

溶液

1 mM柠檬酸钠溶液,pH 6.4

浓度

1.0 mg/mL

应用

报告基因


文献引用:


Li, Bin; Luo, Xiao; Deng, Binbin; Giancola, JoLynn B.; McComb, David W.; Schmittgen, Thomas D.; Dong, Yizhou . Effects of local structural transformation of lipid-like compounds on delivery of messenger RNA.


Marceau, Caleb D.; Puschnik, Andreas S.; Majzoub, Karim; Ooi, Yaw Shin; Brewer, Susan M.; Fuchs, Gabriele; Swaminathan, Kavya; Mata, Miguel A.; Elias, Joshua E.; Sarnow, Peter; Carette, Jan E. . Genetic dissection of Flaviviridae host factors through genome-scale CRISPR screens.


Mahiny, Azita Josefine; Karikó, Katalin . Measuring Hematocrit in Mice Injected with In Vitro-Transcribed Erythropoietin mRNA.


Luo, X.; Li, B.; Zhang, X.; Zhao, W.; Bratasz, A.; Deng, B.; McComb, D. W.; Dong, Y. . Dual-functional lipid-like nanoparticles for delivery of mRNA and MRI contrast agents.


Li, Jiahe; Wang, Wade; He, Yanpu; Li, Yingzhong; Yan, Emily Z.; Zhang, Ketian; Irvine, Darrell J.; Hammond, Paula T. . Structurally Programmed Assembly of Translation Initiation Nanoplex for Superior mRNA Delivery.


Verbeke, Rein; Lentacker, Ine; Wayteck, Laura; Breckpot, Karine; Van Bockstal, Mieke; Descamps, Benedicte; Vanhove, Christian; De Smedt, Stefaan C.; Dewitte, Heleen . Co-delivery of nucleoside-modified mRNA and TLR agonists for cancer immunotherapy: Restoring the immunogenicity of immunosilent mRNA.


Prieve, Mary G.; Harvie, Pierrot; Monahan, Sean D.; Roy, Debashish; Li, Allen G.; Blevins, Teri L.; Paschal, Amber E.; Waldheim, Matt; Bell, Eric C.; Galperin, Anna; Ella-Menye, Jean-Rene; Houston, Michael E. . Targeted mRNA Therapy for Ornithine Transcarbamylase Deficiency.


Schoellhammer, Carl M.; Lauwers, Gregory Y.; Goettel, Jeremy A.; Oberli, Matthias A.; Cleveland, Cody; Park, June Y.; Minahan, Daniel; Chen, Yiyun; Anderson, Daniel G.; Jaklenec, Ana; Snapper, Scott B.; Langer, Robert; Traverso, Giovanni . Ultrasound-Mediated Delivery of RNA to Colonic Mucosa of Live Mice.


Jiang, Yuhang; Gaudin, Alice; Zhang, Junwei; Agarwal, Tushar; Song, Eric; Kauffman, Amy C.; Tietjen, Gregory T.; Wang, Yongheng; Jiang, Zhaozhong; Cheng, Christopher J.; Saltzman, W. Mark . A 'top-down' approach to actuate poly(amine-co-ester) terpolymers for potent and safe mRNA delivery.


Ball, Rebecca L.; Hajj, Khalid A.; Vizelman, Jamie; Bajaj, Palak; Whitehead, Kathryn A. . Lipid Nanoparticle Formulations for Enhanced Co-delivery of siRNA and mRNA.


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