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Protein A ELISA kit

2021-11-16
浏览次数: 3

Protein A ELISA kit是用于监测残留污染的最灵敏(9.01 pg/ml)比色ELISA。产品货号:ADI-900-057,包装规格为:96 wells。


Protein A ELISA kit是一种比色免疫测定酶免疫分析试剂盒,结果在3小时内即可得出。在450 nm处读取吸光度。纯化人源化单克隆抗体制剂中蛋白质A残留的超灵敏定量(<1 ppm)。该试剂盒可识别四种不同的蛋白A结构。

Protein A ELISA kit
Assay recognition of different Protein A constructs, post boiling. Resulting concentrations were interpolated from kit standard curve. Percent recovery calculated by dividing observed concentration by expected concentration. A: Natural Protein A from S. aureus (Millipore), n=9; B: Recombinant Protein A from E. coli (Repligen), n=9; C: Recombinant Cys-Protein A from E. coli (GE), n=12; and D: Recombinant alkaline-resistant Protein A variant from E. coli (MabSelet SuRe from GE), n=12; graphical data represents statistical mean +/- 1 standard deviation.


产品优点:

1.高灵敏度测量,在纯化的人源化单克隆抗体制剂中仅检测到9.01 pg/ml(<1 ppm)的蛋白A残留

2.确保准确的结果,因为它可以识别蛋白质A的多个结构

3.高通量格式,可在3小时内获得多达37个样品(一式两份)

4.与其他技术相比,该解决方案具有成本效益,每次测试的成本更低

5.用于单克隆抗体制剂中蛋白质A变体的污染分析和测量


-产品信息

灵敏度:9.01 pg/ml (range 15.63 - 1,000 pg/ml)

化验时间:<3 hours

实验应用:ELISA, Colorimetric detection

申请须知:For the quantitative determination of residual protein A contamination in protein A purified IgG preparations from any species.

波长:450 nm

种属反应:Species independent

运输:Blue Ice Not Frozen

长期储存:+4°C

Contents:Microtiter plate, Conjugate, Antibody, Assay buffer 13, Wash buffer concentrate,Standard, TMB Substrate, Stop solution 2

科学背景:Staphylococcus aureus Protein A is a 42 kDa cell wall constituent that is characterized by its binding affinity to the Fc portion of some immunoglobulins, especially the IgG class. The IgG binding domain (domain B) consists of three anti-parallel alpha-helices, the third of which is disrupted when the protein is complexed with Fc. Protein A is commonly used to purify antisera and in commonly used immunodetection and visualization techniques. Protein A participates in a number of protective biological functions including anti-tumor, toxic and carcinogenic activities, thus necessitating the removal of potential Protein A contaminants from antibody preparations for therapeutic use.

产品说明:Assay recognition of different Protein A constructs from natural Protein A from S. aureus (M), recombinant Protein A from E. coli (R), recombinant Cys-Protein A from E. coli (G), and recombinant alkaline-resistant Protein A variant from E. coli (S) were tested and the resulting concentrations were interpolated from kit standard curve and displayed in the figure provided.

注意:本产品仅供科研使用


文献参考:An enhanced regeneration strategy to improve microbial control and prolong resin lifetime for Protein A resin in large-scale monoclonal antibody (mAb) purification: T. Zhou, et al.; J. Biotechnol. 289, 118 (2019), Application(s): ELISA using antibody preparation, Abstract;

Indirect monitoring of protein A biosynthesis in E.coli using potentiometric multisensor system: D. Kirsanov, et al.; Sensors and Actuators B: Chemical 238, 1159 (2017), Application(s): Measurement of Protein A


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