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特异性Fe2+荧光探针 FeRhoNoxTM-1

2021-05-14
浏览次数: 504

Specific Fluorescent probe for Fe2+FeRhoNox™-1 

特异性Fe2+荧光探针 FeRhoNoxTM-1

FeRhoNoxTM-1是特异性检测Fe2+的荧光探针

该探针能够特异性地与高尔基体的Fe2+反应。能检测活细胞高尔基体的Fe2+ 


Features  

产品特征 

●Specific detection of Fe2+ 

● 特异性检测Fe2+ 

●Applicable to live cell imaging of labile Fe2+ 

● 适用于不稳定的Fe2+的活细胞成像 

●For detection of Fe2+ in aqueous media 

● 适合于检测水溶性基质里的Fe2+ 

●For detection of labile iron in living cells 

● 适合于检测活细胞内不稳定的离子 


特异性Fe2+荧光探针 FeRhoNoxTM-1



特异性Fe2+荧光探针 FeRhoNoxTM-1

特异性Fe2+荧光探针 FeRhoNoxTM-1 

enceappears aroResponse of FeRhoNox™-1 to Fe2+. Reacting FeRhoNox™-1 with Fe2+for 1 h at 37℃, peak of the fluorescund 575 nm.

Fe2+detection by FeRhoNox™-1in HepG2 cells. HepG2 cells preincubated with/without Fe(NH4)2(SO4)2(FAS: 100 µM) for 0.5 hrwas incubated with 5 µM of FeRhoNox™-1 for 1 hr.

特异性Fe2+荧光探针 FeRhoNoxTM-1

 特异性Fe2+荧光探针 FeRhoNoxTM-1

The selectivity of FeRhoNox™-1.FeRhoNox™-1 can react with only Fe2+selectively.

Comparison between FeRhoNox™-1’s and Golgi Marker’s staining images. The Merge image shows that FeRhoNox™-1 selectively reacts with Fe2+in Golgi apparatus.

 

Code no. 

Product Name 

Target 

Size

GC 901

FeRhoNox™-1

Fe2+in aqueous media, labile Fe2+in living cells

50μg×10




 特异性Fe2+荧光探针 FeRhoNoxTM-1

Application examples using FeRhoNox™-1 

                                             To HepG2 cell 

特异性Fe2+荧光探针 FeRhoNoxTM-1  

① - Fe2+ : not added Fe2+ 100 μM Ferrous ammoniumsulfate

Bpy: cell permeable
Fe2+ selective chelator
(2,20-bipyridyl).

② +Fe2+ : added Fe2+

③ +Fe2+ +Bpy : added Fe2+ and Bpy(1 mM) as Fe2+ chelator

② shows increasing of FeRhoNox™-1’s fluorescence intensity and

③showsdecreasing of it. These results indicate that FeRhoNox™-1 reacts with Fe2+ selectively.



                                             To HEK293 cell

特异性Fe2+荧光探针 FeRhoNoxTM-1 

①-Fe2+: not added Fe2+ 100 μMFerrous ammonium sulfate

②+Fe2+              : added Fe2+

③+Fe2+ +Bpy: added Fe2+and Bpy(1 mM) as Fe2+ chelator

②shows increasing of FeRhoNox™-1’s fluorescence intensity and

③shows decreasing of it. These results indicate that FeRhoNox™-1 
reacts with Fe2+ selectively.

                                             Detection of internal Fe2+

特异性Fe2+荧光探针 FeRhoNoxTM-1 

Since the fluorescence intensity on 
②appears decreased compared to 
①by adding “Bpy”, FeRhoNox™-1 
detects internal iron(II).




                                                   

                                            详情请联系 GORYO CHEMICAL 全国授权代理-欣博盛生物科技

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