产品描述:
The NaveniBright AP kit is an in situ proximity ligation assay kit. The kit enables chromogenic visualization of protein-protein interactions or post-translational modifications such as phosphorylation and ensures high specificity when localizing single proteins. The kits can be used for cultured cells, fresh-frozen, and FFPE samples. Compatible with traditional immunostaining equipment, the kit uses secondary antibody detection to produce an amplified signal in a chromogenic dot. Visualize the signal with a bright-field microscope. The NaveniBright kit is also available with HRP substrate (Item No. 39213). The kit is based on our Naveni™ Proximity Ligation Technology, with two Navenibodies conjugated to proprietary oligo arms. The technology ensures specific and sensitive detection; learn more about Naveni technology. Included in the kit: • Anti-mouse Navenibody • Anti-rabbit Navenibody • AP substrate • Buffers for blocking and dilutions, and all detection reagents • The Navenibody solution has a working volume of 4000 µl (sufficient for approximately 100 reactions) Not included in the kit: • Mouse and rabbit primary antibodies are not included in the kit. For guidance on selecting a primary antibody for the protein of interest, please contact Cayman's Technical Support team. • Mounting medium: NaveniBright assay require VectaMount® Express Mounting Medium (H-5700-60) from Vector Laboratories • AP blocking solution for quenching Precise target localization with intact tissue morphology Correct localization of proteins is critical for understanding and treating many diseases. The quality of in situ protein detection assays depends mainly on the specificity of the antibodies used against the target. Pathologists require intact cell and tissue morphology to confirm cancer cell type and origin of metastatic cancer. Therefore, the “gold standard” for clinical histopathology is enzyme-based chromogenic immunohistochemistry (IHC) of formalin-fixed paraffin-embedded (FFPE) tissue. However, the quality of the IHC method relies mainly on single antibodies. Many commercial antibodies have low specificity to the targets, generating unspecific background staining resulting in misleading results. The Naveni™ Proximity Ligation Technology is based on dual antibody recognition, increasing the specificity leading to the correct local precision of the target.