产品描述:
The Naveni pY PD1 is an in situ proximity ligation assay kit for the chromogenic detection of phosphorylated PD1. Contrary to traditional immunohistochemistry, the kit gives a high signal-to-noise ratio and has outstanding specificity. The kit has been validated on FFPE tissue samples. The kit is based on our Naveni™ Proximity Ligation Technology, with two Navenibodies conjugated to proprietary oligo arms. The technology ensures specific and sensitive detection; learn more about Naveni technology. The Navenibodies in the Naveni pY PD1 kits target two epitopes on the phosphorylated PD1. One Navenibody target the PD1 protein, and the other Navenibody interacts with phosphorylated tyrosine residues. Probes in close proximity will give a strong and distinct signal, creating a specific and sensitive detection method for phosphorylated PD1. Included in the kit: • Navenibody targeting cytoplasmic human VEGFR2 protein, based on cline D4W2J XP® mAb CST • Navenibody targeting phosphorylated tyrosine residues, based on clone P-Tyr-100 mAb CST • HRP substrate, red precipitating reaction product • Buffers for blocking and dilutions, and all detection reagents • The Navenibody solution has a working volume of 4000 µl (sufficient for approximately 100 reactions) Not included in the kit: • VectaMount® Express Mounting Medium (H-5700-60) from Vector Laboratories (required for optimal signal intensity) • Isopropanol 99.5% • Endogenous horseradish peroxidase quenching solution • TBS and TBS-T-Tris-buffered saline and Tris-buffered saline supplemented with 0.05% Tween, respectively Also available with AP Substrate (Item No. 39211) Elucidate the activation of PD1 in tissue VEGFR2 (VEGF receptor 2) is a receptor for VEGF (vascular endothelial growth factor) and an essential protein in the vasculogenesis and angiogenesis signaling pathway. Elevated VEGF within the tumor microenvironment has shown to be an indicator of poor patient prognosis in malignant cancers. VEGF stimulates cellular responses by binding to tyrosine kinase on VEGFR2, leading to phosphorylation and activation. Quantifying phosphorylated VEGFR2 is important to understanding the VEGF signaling pathway. PD1 activation is an important step in the signaling pathway, yet phosphorylation of PD1 in situ is not well studied, mainly due to lack of specific methods Naveni PTM Post-translational modification (PTM), such as tyrosine phosphorylation (pTyr, pY) of proteins, is essential for several cellular functions and pathophysiological processes. The Naveni PTM product line includes kits for detecting tyrosine phosphorylation of RTKs. Western blot has traditionally been used to study phosphorylation of proteins, but specific tools for quantifying phosphorylation of RTKs in situ have been requested for a long time. The Naveni PTM kits now make it possible to detect and quantify low-abundant phosphorylated RTKs in situ with single-cell resolution.