产品描述:
PuroSPIN™ Genomic DNA Purification Kitis designed to provide simple and easy-to-use method for extraction and purification of genomic DNA from Gram-positive and Gram-negative bacteria, yeast, mammalian cells, whole blood and animal tissues.
The purification technology is based on nucleic acid binding to silica spin-columns, which allows efficient removal of protein, salt and other contaminants. Silica spin columns offer faster, simpler and safer alternative to traditional nucleic acid purification techniques such as phenol-chloroform extraction.
PuroSPIN™ buffer formulations produce highly purified DNA product for downstream applications, such as sequencing, ligation, transfection, Southern blotting, qPCR, or microarray analysis.
PuroSPIN™ columns can bind up to 200 μg of DNA product.
Features:
- Easily purify genomic DNA using silica spin columns
- Simple extraction process with no need for phenol or chloroform
- Specialized and detailed protocols for different sample types
- High-quality extracted genomic DNA can be used for a range of downstream applications such as genomic sequencing, PCR, real-time qPCR, or Southern blotting
Citations:
- Hawke, J.P., Daniel, R., Strother, K., Sokolova, Y., Elliott, J., Carossino, M., Langohr, I., Del Piero, F., López-Porras, A., Heckman, T.I., Soto, E. and Griffin, M.J. (2021), Streptococcus dysgalactiae: A Pathogen of Feral Populations of Silver Carp from a Fish Kill Event. J. Aquat. Anim. Health, 33: 231-242. https://doi.org/10.1002/aah.10138
- Gunn, M.A., Allen, P.J., Graham Rosser, T., Wise, D.J. and Griffin, M.J. (2021), Comparative Mortality of Juvenile Channel and Hybrid Catfish Exposed to Bolbophorus damnificus Cercariae. North Am J Aquaculture, 83: 346-353. https://doi.org/10.1002/naaq.10202
- Gunn, MA, Allen, PJ, Rosser, TG, Wise, DJ, Khoo, LH, Griffin, MJ. Assessment of Bolbophorus damnificus prevalence and cercariae shedding in Planorbella trivolvis populations from catfish aquaculture ponds in Mississippi, USA. J World Aquac Soc. 2021; 52: 395– 404. https://doi.org/10.1111/jwas.12756
- Mackenzie A. Gunn, Matt J. Griffin, Brian D. Ott, T. Graham Rosser, Lester H. Khoo, David J. Wise, Peter J. Allen. Physiological response of channel (Ictalurus punctatus) and hybrid (I. punctatus x I. furcatus) catfish following Bolbophorus damnificus infection. Aquaculture. Volume 563, Part 2, 2023.