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S-Glutathionylated Protein Detection Kit
品牌： Cayman
产品货号： 10010721
规格： 1 ea

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  • 产品名称:
    S-Glutathionylated Protein Detection Kit
  • 别名:
    PSSG
  • 产品描述:
    Glutathione (GSH) is a tripeptide (γ-glutamylcysteinylglycine) widely distributed in both plants and animals.{1813,4672} GSH is involved in maintenance of protein sulfhydryl reduction status.{4669,4670} The concentration of GSH ranges from a few micromolar in plasma to several millimolar in tissues such as liver.{4671,4673} Mixed protein glutathionyl disulfides are a post translational protein modification of growing interest.{11632,17080,15065} Protein S-glutathionylation (PSSG) may modify the activity of a large number of cell proteins, including metabolic, structural, cytoskeletal, and signaling proteins.{16795} PSSG detection methods can employ GSH adduct antibodies, GSH derivatives, and differential labeling systems based on the “Biotin-Switch” method.{15065,13441,12802} Cayman’s S-Glutathionylated Protein Detection Assay Kit provides a convenient method for the direct visualization of S-glutathionylated proteins in whole (permeabilized) cells by flow cytometry and microscopy as well as avidin overlay analysis.{15066} This cell-based assay starts with the modification of protein free-thiols groups followed by enzymatic cleavage of any protein-S-glutathione (PSSG) adducts present in the sample. Biotinylation of the newly-formed protein free-thiols provides the basis for visualization using streptavidin-based colorimetric or fluorescence detection. Reagents are provided to test three sets of 10 samples (most convenient) or up to thirty samples total at once if desired.
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    -20
  • 运输温度:
    -20
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  • 有效期:
    1095
  • 产地:
    美国
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  • 来源:
    Plant/Armoracia rusticana
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