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α-Ketoglutarate Detection Assay Kit
品牌： Cayman
产品货号： 701350
规格： 96 wells

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  • 产品名称:
    α-Ketoglutarate Detection Assay Kit
  • 别名:
    2-Oxoglutarate|α-KG
  • 产品描述:
    Cayman’s α-KG Detection Assay provides fluorometric- or colorimetric-based methods for quantifying α-KG in biological samples such as serum, plasma, urine, and tissue. It can also be utilized to determine intracellular and extracellular α-KG concentrations in cell culture samples. In this assay, alanine transaminase (ALT) deaminates α-KG to form pyruvate.{33073,33074} Pyruvate is then oxidized by pyruvate oxidase to yield acetyl phosphate, hydrogen peroxide (H2O2), and carbon dioxide.{33075} As H2O2 is produced, the cycling mechanism of horseradish peroxidase (HRP) results in the concurrent reduction of H2O2 to H2O and the oxidation of 10-acetyl-3,7-dihydroxyphenoxazine (ADHP) to produce the highly fluorescent compound resorufin.{33076} Resorufin fluorescence is analyzed with an excitation wavelength between 530-540 nm and an emission wavelength between 585-595 nm. Alternatively, the absorbance of resorufin can be measured at 570 nm.
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    -20
  • 运输温度:
    -20
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  • 有效期:
    365
  • 产地:
    美国
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    Animal/Bovine
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