产品描述:
eIF2α activator 2 is an activator of eukaryotic translation initiation factor 2α kinase 1 (EIF2AK1), also known as heme-regulated inhibitor kinase (HRI).{68608} It activates HRI and induces eIF2α phosphorylation in a surrogate cell-based reporter assay. eIF2α activator 2 selectively inhibits the proliferation of CRL-2813 melanoma cells (IC50 = 0.46 µM) over non-cancerous NIH3T3 fibroblasts (IC50 = 3.4 µM). It also selectively inhibits the proliferation of CRL-2813 cells transiently transfected with non-targeted siRNA (IC50 = 0.66 µM) over those transiently transfected with HRI-targeted siRNA (IC50 = 5 µM). eIF2α activator 2 (5 µM) increases mRNA expression and protein levels of the eIF2α target DNA damage-inducible transcript 3 (DDIT3), also known as C/EBP homologous protein (CHOP), in CRL-2813 cells.{68609}