产品描述:
Shield-1 is a protein stabilizing ligand that prevents the proteosomal degradation of proteins fused to FK-506 binding protein 1A (FKBP12) containing a leucine-to-proline substitution at position 106 (FKBP12L106P).{67495} It displays specific binding for the destabilizing domain FKBP12L106P and does not interfere with other measured cell processes. Shield-1 inhibits decreases in fluorescence in NIH3T3 cells stably expressing YFP-FKBP12L106P in a concentration-dependent manner. It increases secretion of IL-2-FKBP12L106P in HCT116 cells stably expressing IL-2-FKBP12L106P when used at a concentration of 1 µM.{67496} In vivo, shield-1 (5 and 10 mg/kg every two days) reduces tumor volume in a mouse xenograft model using HCT116 cells stably expressing IL-2-FKBP12L106P.