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CUTANA™ CUT&Tag Kit with Primer Set 1
品牌： EpiCypher
产品货号： 14-1102
规格： 48 Reactions

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  • 产品名称:
    CUTANA™ CUT&Tag Kit with Primer Set 1
  • 别名:
  • 产品描述:
    The CUTANA™ CUT&Tag Kit offers a comprehensive solution for ultra-sensitive mapping of histone post-translational modifications (PTMs). This kit uses an exclusive Direct-to-PCR strategy to go from cells to PCR amplified sequencing libraries in one tube, bypassing traditional library prep and minimizing sample loss. The protocol is also designed for compatibility with multi-channel pipetting for increased throughput and reproducibility. Positive (H3K27me3) and negative (IgG) control antibodies are paired with the SNAP-CUTANA™ K-MetStat Panel of nucleosome spike-in controls (Figure 2) to continuously monitor workflows and guide troubleshooting. The recommended input for CUT&Tag is 100,000 native nuclei per reaction. Comparable data can be generated down to 10,000 nuclei, and the protocol is also validated for whole cells, cryopreserved samples, and lightly cross-linked nuclei or cells. CUT&Tag provides robust profiling for histone PTMs. For chromatin-associated proteins (e.g. transcription factors), CUTANA™ CUT&RUN is recommended (EpiCypher 14-1048, EpiCypher 14-1001).
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  • 保存温度:
    OPEN KIT IMMEDIATELY and store components at room temperature, 4°C, and -20°C as indicated (see User Manual corresponding to Kit Version 1). Stable for 6 months upon date of receipt.
  • 运输温度:
    DO NOT FREEZE!! Frozen cold packs. Overnight (preferred) or 2-day shipping.
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  • 产品形式:
    Kit contains buffers, enzymes, magnetic beads, control antibodies, spike-in controls, 8-strip tubes, and spin columns necessary to prepare and purify CUT&RUN DNA starting from cells or nuclei. See user manual corresponding to Kit Version 1 for additional materials and equipment required.
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    美国
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