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产品名称:
Streptavidin (FITC)
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别名:
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产品描述:
Streptavidin is a 55 kDa (subunit MW 14 kDa) biotin-binding protein isolated from Streptomyces avidini. Streptavidin is superior to avidin, because it does not contain carbohydrate like avidin and has no net charge at neutral pH. Streptavidin~biotin system is routinely used in Immunohistochemistry (IHC). Extinction Coefficient 1% A280=32.0; Eletrophoretically homogenous, single band. Protein content: > 95%; One mg of Streptavidin is conjugated with FITC, unconjugated Streptavidin is removed. This conjugate of streptavidin is recommended for use with Biotin~conjugated antibodies. Avidin~Biotin conjugated reagents are stable, sensitive and give less background. Fluorophore: Fluorescein isothiocyanate A max=492nm; E=520nm; Fluorophore/Protein: A492nm/A280nm=~1
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反应种属:
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宿主来源:
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实验应用:
FACS, ICC/IF, IHC-P
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免疫原:
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偶联:
FITC
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阳性对照:
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保存温度:
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. Protect from light.
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运输温度:
4°C
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预期分子量:
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产品形式:
Liquid
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存储溶液:
10mM Phosphate (pH7.4), 150mM NaCl, 10mg/ml BSA
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有效期:
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产地:
美国
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功能与背景:
Streptavidin is a 55 kDa (subunit MW 14 kDa) biotin-binding protein isolated from Streptomyces avidini. Streptavidin is superior to avidin, because it does not contain carbohydrate like avidin and has no net charge at neutral pH. Streptavidin~biotin system is routinely used in Immunohistochemistry (IHC). Extinction Coefficient 1% A280=32.0. Purity: > 99% pure by HPLC; Eletrophoretically homogenous, single band. Protein content: > 95%; Activity 14.8 units/ mg, one unit bunds 1.0 microgram of d-biotin; Protease activity: 0.000018 units/mg; Isoelectric point: 7.0 +/- determined by Isoelectric focusing. One mg of Streptavidin is conjugated with FITC, unconjugated Streptavidin is removed. This conjugate of streptavidin is recommended for use with Biotin~conjugated antibodies. Avidin~Biotin conjugated reagents are stable, sensitive and give less background. Fluorophore: Fluorescein isothiocyanate A max=492nm; E=520nm; Fluorophore/Protein: A492nm/A280nm=~1
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样本类型:
10mM Phosphate (pH7.4), 150mM NaCl, 10mg/ml BSA
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