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Streptavidin, AMCA, 1 mg/链霉亲和素,AMCA标记
品牌: Vectorlabs
产品货号: SA-5008-1
规格: 1 mg

报价:
折后价:

数量: - +
产品详情
* 以下信息仅供参考,详情请以原厂网站为准
  • 产品名称:
    Streptavidin, AMCA, 1 mg/链霉亲和素,AMCA标记
  • 别名:
    链霉亲和素,AMCA标记
  • 产品描述:
    AMCA Streptavidin can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry. Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.AMCA Streptavidin is produced by conjugating streptavidin with a coumarin fluorescent dye, 7-amino-4-methylcoumarin-3-acetic acid. This derivative excites in the ultraviolet (350 nm) and emits in the visible (450 nm) producing an intense blue fluorescence. Most paraffin-embedded tissues have little autofluorescence under those conditions. AMCA Streptavidin may provide an advantage in many labeling situations over conjugates of fluorescein and related dyes. AMCA Streptavidin can be used on frozen or paraffin-embedded sections as a single label or in combination with other fluorescent conjugates for multiple labeling studies.
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  • 实验应用:
    Immunofluorescence,In situ hybridization,Flow Cytometry/Cell Separation
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  • 推荐稀释度:
    For diluting this product, we recommend a HEPES- or biocarbonate-buffered saline solution, approximately pH 8.2.Avoid using RPMI 1640 or other biotin-containing solutions as diluents.Serum also can contain biotin and should not be added to diluents. The recommended concentration range for use is 10-30µg/ml.
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  • 偶联:
    AMCA
  • 产品浓度:
    1.0 mg/ml
  • 表达系统:
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  • 保存温度:
    2° - 8°C – Do Not Freeze
  • 运输温度:
    Ambient
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  • 产品形式:
    Concentrate
  • 存储溶液:
    10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide
  • 有效期:
  • 产地:
    美国
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  • 样本类型:
    10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide
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