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LDH Cytotoxicity Assay Kit
品牌: Cayman
产品货号: 601170-480 wells
规格: 480 wells

报价:
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数量: - +
产品详情
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  • 产品名称:
    LDH Cytotoxicity Assay Kit
  • 别名:
    Lactate Dehydrogenase Cytotoxicity Assay Kit
  • 产品描述:
    Necrosis is accompanied by mitochondrial swelling and increased plasma membrane permeability, while apoptosis involves an articulated breakdown of the cell into membrane-bound apoptotic bodies. Lactate dehydrogenase (LDH) is a soluble cytosolic enzyme that is released into the culture medium following loss of membrane integrity resulting from cell- or compound-induced cytotoxicity. LDH activity, therefore, can be used as an indicator of cell membrane integrity and serve as a general means to assess cell viability by measuring plasma membrane permeability. Cayman's LDH Cytotoxicity Assay Kit measures cell death in response to chemical compounds or environmental factors using a coupled two-step reaction. In the first step, LDH catalyzes the reduction of NAD+ to NADH and H+ by oxidation of lactate to pyruvate. In the second step of the reaction, diaphorase uses the newly-formed NADH and H+ to catalyze the reduction of a tetrazolium salt (INT) to highly-colored formazan which absorbs strongly at 490-520 nm.
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    -20
  • 运输温度:
    -20
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  • 有效期:
    365
  • 产地:
    美国
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    Animal/Rabbit
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