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Annexin V-FITC Apoptosis Detection Kit
品牌: GeneTex
产品货号: GTX85591
规格: 100assay

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  • 产品名称:
    Annexin V-FITC Apoptosis Detection Kit
  • 别名:
  • 产品描述:
    Annexin V Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, cells translocate the membrane phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a high affinity for PS. The one-step staining procedure takes only 5-15 minutes. Detection can be analyzed by flow cytometry or fluorescence microscopy. The kit can differentiate apoptosis vs necrosis when performing both Annexin V-FITC and PI staining.
    Detection method - Flow cytometry (Ex = 488 nm; Em = 530 nm) and fluorescence microscopy
    Sample type - Living cells (suspension and adherant)
    Species reactivity - Mammalian
    Applications - Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

    Features and Benefits
    Simple one step staining procedure in 5-15 minutes
    Fast and convenient
    Kit can differentiate apoptosis vs necrosis when performing both Annexin V-FITC and PI staining

    Kit Contents:
    Annexin V-FITC - Formulated in 0.01 M PBS pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
    1X Binding Buffer - Formulated in 0.01 M PBS pH 7.4, 150 mM NaCl. (Caution: Propidium Iodide Solution is toxigenic and mutagenic; Please handle with care.)
    Propidium Iodide (PI) - No preservative added

    Other Materials and Solutions Required:
    Fluorescence Microscope or Flow Cytometer (Capable of FITC and Propidium Iodide Detection)
    Microcentrifuge
    Adjustable Pipettors (100-1000 μl, 10-200 μl, and 1-20 μl)
    Ice Bucket and Ice
    PBS Buffer
    Microcentrifuge Tubes
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    FACS, ICC/IF
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  • 保存温度:
    Stable when stored dark at 2º-8ºC. Do Not Freeze.
  • 运输温度:
    4°C
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  • 产地:
    美国
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