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NAPRTase (human) (rec.) (His)
品牌： Adipogen
产品货号： AG-40T-0105-C050
规格： 50 ug

报价：
折后价：

数量： - +
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  • 产品名称:
    NAPRTase (human) (rec.) (His)
  • 别名:
    Nicotinate Phosphoribosyltransferase; FHA-HIT-interacting Protein; FHIP; EC 2.4.2.11
  • 产品描述:
    Human nicotinate phosphoribosyltransferase (NAPRTase) is localized in the cytoplasm and is involved in biological processes such as NAD biosynthetic and metabolic processes, nicotinamide metabolic process, nicotinate nucleotide salvage, response to oxidative stress and water-soluble vitamin metabolic process. It functions by catalyzing the conversion of nicotinic acid (NA) to NA mononucleotide (NaMN) and is essential for NA to increase cellular NAD levels and prevent oxidative stress of the cells. It is a crucial factor in the NAD+ biosynthesis pathway. Catalytic activity: Beta-nicotinate D-ribonucleotide + diphosphate = nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate.
  • 反应种属:
  • 宿主来源:
    E. coli
  • 实验应用:
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  • 推荐稀释度:
  • 免疫原:
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  • 偶联:
  • 产品浓度:
    0.5mg/ml (Lot specific)
  • 表达系统:
  • 阳性对照:
  • 保存温度:
    -80°C
  • 运输温度:
    DRY ICE
  • 细胞定位:
  • 预期分子量:
    ~58.0kDa
  • 产品形式:
    liquid
  • 存储溶液:
    Liquid. In 50mM HEPES/KOH pH 7.5 containing 300mM potassium chloride and 10mM DTT.
  • 有效期:
    Stable for at least 6 months after receipt when stored at -80°C.
  • 产地:
    瑞士
  • 外观:
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  • 检测方法:
  • 样本类型:
    Liquid. In 50mM HEPES/KOH pH 7.5 containing 300mM potassium chloride and 10mM DTT.
  • 产品特点:
  • 纯度:
    >98% (SDS-PAGE)
  • 生物活性:
    0.043µmol/min/mg: hNAPRTase activity was measured by a custom coupled enzymatic assay which uses a secondary enzyme for the quantification of the PRPP consumed by hNAPRTase. The reaction buffer contained 50 mM Hepes pH 7.5, 500 µM PRPP, 100 µM nicotinic acid, 20 mM MgCl2, 2.5 mM DTT and hNAPRTase at 5 µM, 25°C, spectrophotometrical monitoring.
  • 内毒素水平:
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