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产品名称:
ConA Lectin
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别名:
Concanavalin A , Concanavalin A , Canavalia ensiformis , Canavalia ensiformis lectin , ConA lectin , Canavalia ensiformis , ConA , Concanavalin A lectin , Con A lectin
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产品描述:
Concanavalin A is a lectin protein (MW 104kDa), homotetramer 26 kDa; originally extracted from the jack-bean, Canavalia ensiformis. It binds specifically to certain structures found in various sugars α-mannosyl and α-glucosyl residues in glycoproteins. It was the first lectin to be available on a commercial basis and is widely used in biology and biochemistry to characterize glycoproteins and other sugar-containing entities. It is also used to purify macromolecules in lectin affinity chromatography. Concanavalin A interacts with diverse receptors containing mannose carbohydrates (serum and membrane glycoproteins). ConA agglutinate strongly erythrocytes without being blood group specific. Normal cell reagglutinated after trypsinisation. ConA is noy only a lymphocyte mitogen and reacts with many bacteria, like E. coli Dictyostelium discoideum et B. substilis, but also widely believed to be involved in the interaction between alpha-mannosyl oligosaccharides on the surface of the HIV virus and the human T cell lymphocyte.
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反应种属:
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宿主来源:
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实验应用:
Conjugation, Purification
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纯化方式:
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偶联:
Unconjugated
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产品浓度:
10 mg/ml (Please refer to the vial label for the specific concentration.)
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表达系统:
Native Protein
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阳性对照:
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保存温度:
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
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运输温度:
4°C
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产品形式:
Liquid
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存储溶液:
10mM Bicarbonate, 150mM NaCl, 0.1mM Calcium Chloride, 0.01mM Manganese Chloride (pH8.0). (Con A has an Isoelectric point of about pH4.5-5.5, requires calcium or manganese ions at each of its four saccharide binding sites; These ions should be used in buffer. DO NOT USE Phosphate buffer for dilution of this lectin as it will decrease the activity of lectin), 0.05% Sodium azide.
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有效期:
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产地:
美国
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样本类型:
10mM Bicarbonate, 150mM NaCl, 0.1mM Calcium Chloride, 0.01mM Manganese Chloride (pH8.0). (Con A has an Isoelectric point of about pH4.5-5.5, requires calcium or manganese ions at each of its four saccharide binding sites; These ions should be used in buffer. DO NOT USE Phosphate buffer for dilution of this lectin as it will decrease the activity of lectin), 0.05% Sodium azide.
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