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产品名称:
Alpha Synuclein Protein
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别名:
A53T mutant alpha synuclein, A53T mutated SNCA, A53T Alpha synuclein monomer, Ala53thr mutant alpha synuclein, Active Alpha synuclein protein, Alpha-synuclein protein, Non-A beta component of AD amyloid protein, Non-A4 component of amyloid precursor protein, NACP protein, SNCA protein, NACP protein, PARK1 protein, Alpha synuclein monomers, SYN protein, Parkinson disease familial 1 Protein
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产品描述:
Human Recombinant A53T Mutant Alpha Synuclein Protein Monomers (Type 1)
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反应种属:
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宿主来源:
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实验应用:
WB | SDS-PAGE | In vivo assay | In vitro assay
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靶标/特异性:
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同种型:
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推荐稀释度:
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免疫原:
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免疫原种属:
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克隆性:
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克隆号:
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纯化方式:
Ion-exchange Purified
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偶联:
No tag
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产品浓度:
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表达系统:
E. coli
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阳性对照:
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保存温度:
-80ºC
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运输温度:
Dry Ice. Shipping note: Product will be shipped separately from other products purchased in the same order.
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细胞定位:
Cytoplasm | Membrane | Nucleus
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预期分子量:
~14.46 kDa
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产品形式:
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存储溶液:
PBS pH 7.4
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有效期:
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产地:
加拿大
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外观:
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生产商:
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功能与背景:
Alpha-Synuclein (SNCA) is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals (1). Alpha-synuclein is highly expressed in the mitochondria of the olfactory bulb, hippocampus, striatum and thalamus (2). Functionally, it has been shown to significantly interact with tubulin (3), and may serve as a potential microtubule-associated protein. It has also been found to be essential for normal development of the cognitive functions; inactivation may lead to impaired spatial learning and working memory (4). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimers disease amyloid plaque, and a major component of Lewy body inclusions, and Parkinson's disease. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin (5, 6). The A53T mutation is a missense point mutation where alanine is replaced by threonine at the 53rd amino acid. This mutation has been linked to early-onset Parkinson's Disease and increased rates of alpha synuclein fibrillization.
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URL:
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灵敏度:
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检测范围:
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检测方法:
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样本类型:
PBS pH 7.4
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产品特点:
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纯度:
>95%
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生物活性:
100 µM A53T alpha synuclein protein monomer (SPR-325) seeded with 10 uM A53T alpha synuclein protein PFF (SPR-326) in 25 µM Thioflavin T (PBS pH 7.4, 100 µl reaction volume) generated a fluorescence intensity of 28 000 Relative Fluorescence Units after incubation at 37°C with shaking at 600 rpm for 56 hours. Fluorescence was measured by excitation at 450 nm and emission at 485 nm on a Molecular Devices Gemini XPS microplate reader.
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内毒素水平:
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来源:
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表达方式:
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Accession #:
NP_000336.1
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CAS号:
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分子式:
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溶解度:
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PubChem CID: