Tim-4 (mouse):Fc (human) (rec.) (Biotin) - 深圳欣博盛生物科技有限公司

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Tim-4 (mouse):Fc (human) (rec.) (Biotin)

品牌： Adipogen

产品货号： AG-40B-0180B-C010

规格： 10 ug

报价：

折后价：

数量： - +

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产品名称:

Tim-4 (mouse):Fc (human) (rec.) (Biotin)
别名:

TIM4; TIMD4; T Cell Immunoglobulin and Mucin Domain-containing Protein 4
产品描述:

Extracellular vesicles (EVs) are released by a variety of cells into the cellular microenvironment and have the natural ability of delivering different cargos and carry bioactive molecules such as non-coding RNA, miRNAs, genomic DNA, lipids, growth factors and signaling molecules. EVs can be divided into exosomes (30-100nm), microvesicles (100-1000nm) and apoptotic bodies (>1000nm). EVs play substantial roles not only in the regulation of normal physiological processes but also in disease pathogenesis and their cargo reflects the status of parental cells at the time of secretion. Various studies are currently being conducted to develop therapeutic and diagnostic methods targeting or utilizing EVs. Therefore, developing ideal methods for isolating and quantifying EVs is an active area of research. EVs express phosphatidylserine (PS) on their outer lipid bilayer. Tim-4 (T cell immunoglobulin and mucin domain-containing protein 4) is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily and TIM family. Tim-4 contains one Ig-like V-type (immunoglobulin-like) domain. It is expressed on dendritic cells and macrophages. Tim-4 plays an important role in the proliferation of T helper type 2 (Th2) cells. Tim-4 binds to phosphatidylserine (PS) on the surface of apoptotic cells in a calcium-dependent manner and mediates phagocytosis of apoptotic cells. EV membranes are rich in phosphatidylserine (PS) and Tim-4 binds to PS on the surface of EVs. A new protocol from the group of Prof. Rikinari Hanayama describes an affinity-based method for isolating EVs using streptavidin magnetic beads conjugated with Tim-4-biotin to capture EVs in a calcium-dependent manner [2]. This new protocol could replace ultracentrifugation, that is the most commonly used method for purifying EVs. This new Tim-4-dependent method gives good yield, high purity and allows isolation of all populations of EVs compared to other approaches (ultracentrifugation, PEG precipitation or selected antibodies immunoprecipitation). See Reference 2 for a complete protocol (Download available).
反应种属:
宿主来源:

CHO cells
实验应用:
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同种型:
推荐稀释度:
免疫原:
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纯化方式:
偶联:

Biotin
产品浓度:

0.1mg/ml after reconstitution
表达系统:
阳性对照:
保存温度:

-20°C
运输温度:

BLUE ICE
细胞定位:
预期分子量:

~95kDa (SDS-PAGE)
产品形式:

solid
存储溶液:

Lyophilized. Contains PBS.
有效期:

Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
产地:

瑞士
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样本类型:

Lyophilized. Contains PBS.
产品特点:
纯度:

>95% (SDS-PAGE)
生物活性:

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. The Tim-4 (mouse):Fc (human) (rec.) (Biotin) is used with magnetic beads to isolate extracellular vesicles. 120ng of the protein is sufficient to isolate 1010 particles in a calcium-dependent manner.
内毒素水平:

<0.05EU/µg protein (LAL test).
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