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HIF-1α Transcription Factor Assay Kit
品牌： Cayman
产品货号： 10006910
规格： 96 wells

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数量： - +
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  • 产品名称:
    HIF-1α Transcription Factor Assay Kit
  • 别名:
    ARNT-interacting Protein|Hypoxia-Inducible Factor-1α
  • 产品描述:
    The HIF (hypoxia-inducible factor) transcription factor is a member of the basic-helix-loop-helix (bHLH) family of transcription factors and plays an important role in maintaining cellular oxygen homeostasis.{11044,12428} HIF-1α has emerged as an important drug target in breast and prostate cancer, cardiovascular disease, and ischemia.{10485,10724,11835} Cayman’s HIF-1α Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysate. A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) consensus sequence containing the HIF-1α response element is immobilized to the wells of a 96-well plate. HIF-1α contained in a nuclear extract or whole cell lysates, binds specifically to the HIF-1α response element. The HIF transcription factor complex is detected by addition of a specific primary antibody directed against HIF-1α. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm.
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  • 保存温度:
    -80
  • 运输温度:
    -80
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  • 有效期:
    365
  • 产地:
    美国
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