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His Tag Antibody / Histidine
品牌： NSJ Bio
产品货号： V8566-100UG
规格： 100 ug

报价：
折后价：

数量： - +
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  • 产品名称:
    His Tag Antibody / Histidine
  • 别名:
  • 产品描述:
    It recognizes both the free and protein-conjugated (either soluble or cell bound) form of hexa-histidine. This MAb is highly specific to hexa-histidine and shows no cross-reaction with other structurally related compounds. It has a very high affinity for hexa-histidine and is excellent for use in various techniques. His-Tag Antibody detects recombinant proteins containing the 6xHis epitope tag. The antibody recognizes the 6xHis-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged proteins biochemical properties. A variety of plasmids contain DNA that encodes an amino-terminal tag consisting of six histidine (6xHis) residues followed by an extended multiple cloning site. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni2+ affinity resins and purification by single step chromatography. As is the case with other protein tag systems, this polyhistidine tag can often be cleaved at sites recognized by proteases such as thrombin and enterokinases to isolate the protein of interest.
  • 反应种属:
    All species
  • 宿主来源:
    Mouse
  • 实验应用:
    WB, FACS, IF, IP
  • 靶标/特异性:
  • 同种型:
    Mouse IgG1, kappa
  • 推荐稀释度:
  • 免疫原:
    Hexa-histidine tagged human recombinant protein was used as the immunogen for the Histidine antibody.
  • 免疫原种属:
  • 克隆性:
    Monoclonal (mouse origin)
  • 克隆号:
  • 纯化方式:
    Protein G affinity chromatography
  • 偶联:
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  • 保存温度:
    Store the Histidine antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
  • 运输温度:
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  • 产品形式:
  • 存储溶液:
    0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
  • 有效期:
  • 产地:
    美国
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  • 样本类型:
    0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
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