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Maltose Binding Protein Antibody / malE / MBP tag
品牌： NSJ Bio
产品货号： V8292-100UG
规格： 100 ug

报价：
折后价：

数量： - +
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  • 产品名称:
    Maltose Binding Protein Antibody / malE / MBP tag
  • 别名:
  • 产品描述:
    Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage; such expression vectors frequently encode hybrid fusion proteins consisting in part of prokaryotic and in part, eukaryotic specified proteins. One such system utilizes maltose binding protein (MBP), the 370 amino acid product of the E. coli mal E gene. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be Purified in a one step procedure by affinity chromatography crosslinked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by Western Blot Analysis or immunoprecipitation using antibodies specific for the MBP-tag. Expression systems utilizing the MBP fusion tag include pCG-806fx and pMal vectors.
  • 反应种属:
    MBP fusion proteins
  • 宿主来源:
    Mouse
  • 实验应用:
    IF, WB
  • 靶标/特异性:
  • 同种型:
    Mouse IgG1, kappa
  • 推荐稀释度:
  • 免疫原:
    MOS maltose binding protein fusion protein was used as the immunogen for this MBP tag antibody.
  • 免疫原种属:
  • 克隆性:
    Monoclonal (mouse origin)
  • 克隆号:
  • 纯化方式:
    Protein G affinity chromatography
  • 偶联:
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  • 保存温度:
    Store the MBP tag antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
  • 运输温度:
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  • 产品形式:
  • 存储溶液:
    0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
  • 有效期:
  • 产地:
    美国
  • 外观:
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  • 样本类型:
    0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
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